Prerequisites for Module
Population Genetics (AS4020) or equivalent.
Corequisite Modules
Forensic DNA Analysis (AS4070) or equivalent.
Precluded Modules
Refer to Definitive Course Document.
Aims of Module
To provide the concepts and principles underlying the forensic analysis of DNA.
Learning Outcomes for Module
On completion of this module, students are expected to be able to:
| 1. |
Perform effectively and safely a range of advanced molecular biology techniques, involved with the analysis and interpretation of DNA evidence.
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| 2. |
Evaluate critically experimental data and idenitfy sources of error and uncertainty.
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Indicative Module Content
Extraction and preparation of DNA samples from biological samples (e.g. blood, semen, hair, saliva, tissues). Analysis of DNA by gel electophoresis. Restriction digestion, analysis of DNA fragments. Amplification of DNA samples by the PCR and the analysis of fluorescent PCR products, fluorescent gel scanning, capillary electophoresis. Evaluation of DNA evidence, contamination problems. Analysis and interpretation of anonymous data supplied by Forensic Laboratories using appropriate software.
Indicative Student Workload
Contact Hours
| Full Time | Laboratory
| 40 | Demonstrations
| 10 | Supervised Assessments
| 5 |
Directed Study
| | Case Studies
| 20 |
Private Study
| | Private Study
| 75 |
| Mode of Delivery
The course is laboratory based and will also involve demonstrations of techniques, library-based and computer-based investigations.
Assessment Plan
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Learning Outcomes Assessed
| | Coursework | 1,2
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The assessment is based on laboratory work. The student's performance in the laboratory is continuously assessed through a number of criteria: safe use of instruments, laboratory diary inspection, formal written report, evaluation of quality, accuracy and professional conduct, critically presenting reporting and defending results and conclusions.
Indicative Bibliography
| 1. | Polymerase Chain Reaction: A Practical Approach, Volume 1, M J McPherson, P Quirke, G R Taylor (Eds), (1994).
| | 2. | Polymerase Chain Reaction: A Practical Approach, Volume 2, M J McPherson, B D Hames, G R Taylor (Eds), (1995).
| | 3. | Forensic DNA Profiling Protocols. Methods in Molecular Biology, Volume 98, P J Lincoln, J Thomson, J Thomson (Eds), Humana Press (1998).
| | 4. | Forensic DNA Typing: Biology and Technology behind STR Markers, M Butler, Academic Press (2000).
| | 5. | Evaluation of Forensic Science DNA Evidence: Update on Evaluating DNA Evidence, National Research Council, Committee on DNA Technology in Forensic Science, (1996).
| | 6. | Interpreting DNA Evidence, Statistics for Forensic Scientists, I W Evett and B S Weir, Sinaner Associates (1998).
| | 7. | School of Life Sciences Laboratory Manual.
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