Prerequisites for Module
Successful completion of Stage 2 of the course, or equivalent.
Corequisite Modules
Refer to Definitive Course Document.
Precluded Modules
Refer to Definitive Course Document.
Aims of Module
To provide the student with the ability to examine the major elements associated with recombinant DNA technology and human genetics and heredity.
Learning Outcomes for Module
On completion of this module, students are expected to be able to:
| 1. |
Provide details of molecular cloning procedures, including restriction endonucleases, expression vectors and recombinant selection systems.
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| 2. |
Describe the methods employed for DNA, RNA and protein analysis, including gel electrophoresis, hybridisation, polymerase chain reaction and sequencing.
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| 3. |
Explain the application of Mendelian principles in genetic investigation.
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| 4. |
Predict genetic outcomes of congenital and inherited disorders.
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Indicative Module Content
Molecular Biology: restriction endonucleases; recombinant DNA formation; ligation; cloning strategies; bacterial expression vectors - plasmids, phage, selection of recombinant clones; mammalian expression vectors; transfection; genetic immunological and nucleic acid based detection methods; mRNA, cDNA, PCR, gel electrophoresis; Southern, Northern and Western hybridisation; sequencing.
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Human Genetics: Mendelian principles of inheritance - segregation and independent assortment; genetic probability; complex dominance - ABO blood groups; sex-linked disorders; mutagens and cancer inheritance - Downs syndrome; genetic screening; gene therapy.
Indicative Student Workload
Contact Hours
| Full Time | Lectures
| 36 | Tutorials/Seminars
| 12 | Supervised Assessments
| 3 |
Directed Study
| | Directed Study
| 42 |
Private Study
| | Private Study
| 57 |
Mode of Delivery
No Information.
Assessment Plan
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Learning Outcomes Assessed
| | Coursework | 1,2,3,4
| | Examination | 1,2,3,4
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The examination will consist mainly of extended response questions.
Indicative Bibliography
| 1. | T A Brown. Gene Cloning and DNA Analysis, (4th Edition), Blackwell Publishing (2001).
| | 2. | Primrose S.B. et al. Principles of Gene Manipulation - an introduction to genetic engineering (6th Edit.), Blackwell Publishing (2002)
| | 3. | R. Lewis, Human Genetics: Concepts and Applications, (5th Edition) McGraw Hill (2003).
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