Prerequisites for Module
AS1009 Data Handling Skills or equivalent.
Corequisite Modules
None.
Precluded Modules
None.
Aims of Module
To provide students with a broad understanding of the principles of bioanalytical instrumentation and an appreciation of their uses.
Learning Outcomes for Module
On completion of this module, students are expected to be able to:
| 1. |
Explain the theoretical principles and applications of immunological techniques.
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| 2. |
Explain the theoretical principles of radioactivity and appreciate the uses of radioisotopes.
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| 3. |
Explain the theoretical principles of centrifugal techniques and appreciate the scope of their applications
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| 4. |
Explain the theoretical principles of electroanalytical and electrophoretic technques and some of their applications
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| 5. |
Perform relevant laboratory experiment using the techniques listed in LOs 1-4 to reflect the practical nature of the Module Title.
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Indicative Module Content
Methods: Basic structure of antibodies, polyclonal and monoclonal antibodies production & uses. Agglutination & precipitation tests. Imunodiffusion, immunelectrophoretic, radioimmunological, complement-based and enzyme-linked immunosorbent assays.
Radioactive Isotopes and their Uses: Radioactive decay, measurement of radioactivity, biologically useful radioisotopes and radiological protection.
| Centrifugation: Principles, types of centrifuges, separation methods and safety.
Electrophoresis: Principles, protein separation techniques, detection and applications.
Advanced Electrophoretic Techniques: IEF and 2-D.
Overview of Electroanalytical Techniques: Potentiometry, ion-selective electrodes, voltammetric methods, oxygen electrodes, glucose electrodes
Indicative Student Workload
Contact Hours
| Full Time | Lectures
| 30 | Workshops
| 10 | Tutorials
| 10 | Assessment
| 3 |
Directed Study
| | Directed Study
| 30 |
Private Study
| | Private Study
| 67 |
Mode of Delivery
This course is delivered mostly by formal lectures and workshops with appropriate tutorial support.
Assessment Plan
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Learning Outcomes Assessed
| | Coursework | 4
| | Examination | 1,2,3,5
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The coursework will be a team-based oral and written presentation.
The examination is closed book.
Indicative Bibliography
| 1. | REED, R., HOLMES, D., WEYERS, J. AND JONES, A., 2003. Practical Skills in Biomolecular Sciences. 2nd ed. Pearson.
| | 2. | HOLME, D.J. AND PECK, H., 1998. Analytical Biochemistry. 3rd ed. Longman.
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