Module Title
Bioanalytical Techniques
Keywords
Immunological, radioisotopic, centrifugal, electrophoretic, electroanalytical and chromatographic techniques, Certificate of Competence Portfolio of Registration. |
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Prerequisites for Module
None in addition to standard entrance requirements.
Corequisite Modules
None.
Precluded Modules
None.
Aims of Module
To provide students with a broad understanding of the principles of bioanalytical instrumentation and to provide an appreciation of their uses.
Learning Outcomes for Module
On completion of this module, students are expected to be able to:
| 1. |
Explain the theoretical principles and applications of immunological techniques.
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| 2. |
Explain the theoretical principles of radioactivity and appreciate the uses of radioisotopes.
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| 3. |
Explain the theoretical principles of centrifugation techniques and appreciate the scope of their applications.
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| 4. |
Explain the theoretical principles of electrophoretic and some electroanalytical technques and some of their applications.
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| 5. |
Explain the theoretical principles and applications of a range of chromatographic techniques.
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Indicative Module Content
Immunological Methods: Basic structure of antibodies, polyclonal and monoclonal antibodies, production & uses. Agglutination & precipitation tests. Imunodiffusion, immunelectrophoretic, radioimmunological, complement-based and enzyme-linked immunosorbent assays.
Radioactive Isotopes and their Uses: Radioactive decay, measurement of radioactivity, biologically useful radioisotopes and radiological protection.
Centrifugation: Principles, types of centrifuges, separation methods and safety.
Electrophoresis: Principles, protein and nucleic acid separation techniques, detection and applications.
| Advanced Electrophoretic Techniques: Isoelectric focusing and 2-D gel electrophoresis.
Overview of Electroanalytical Techniques: Potentiometry, ion-selective electrodes, voltammetric methods, oxygen electrodes, glucose electrodes.
Chromatographic Separations: gas, high-performance liquid and thin-layer chromatography, FPLC and capillary elecrophoresis.
Indicative Student Workload
Contact Hours
| Full Time | Lectures
| 30 | Workshops
| 10 | Tutorials
| 10 | Assessment
| 3 |
Directed Study
| | Directed Study
| 30 |
Private Study
| | Private Study
| 67 |
Mode of Delivery
This course is delivered by formal lectures, directed study and supporting workshops with appropriate tutorial support.
Assessment Plan
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Learning Outcomes Assessed
| | Component 1 | 2,3,4,5
| | Component 2 | 1
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The examination will be closed book.
The coursework is on Immunological Methods.
Indicative Bibliography
| 1. | REED, R., HOLMES, D., WEYERS, J. AND JONES, A., 2003. Practical Skills in Biomolecular Sciences. 2nd ed. Pearson.
| | 2. | HOLME, D.J. AND PECK, H., 1998. Analytical Biochemistry. 3rd ed. Longman.
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Additional Notes
This module covers, at least in part, the following Health Professions Council Standards of Proficiency for Biomedical Scientists (035/SOP/BMS/A5 July 2004): 3a.1
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