Prerequisites for Module
None, in addition to course entry requirements.
Corequisite Modules
None.
Precluded Modules
None.
Aims of Module
To enable students to acquire the knowledge and practical experience of the handling of animal cells using sterile and safe techniques.
Learning Outcomes for Module
On completion of this module, students are expected to be able to:
| 1. |
Recognise and describe the basic principles of aseptic techniques as they apply to the elimination of microbial contamination, for animal cell/tissue culture.
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| 2. |
Demonstrate laboratory competence in performing tasks relating to Learning Outcome 1.
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| 3. |
Perform diagnostic and investigative applications of cell culture techniques using cell culture systems.
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Indicative Module Content
Sterile technique and management of microbial contamination: sterile techniques relating to animal tissue and cell culture, primary vs secondary cultures, animal tissues and organs, applications of cell and tissue cultures, and animal cell culture systems. Measurement of growth in culture systems: solid and liquid media, batch and continuous culture, growth measurement techniques, bioassays.
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Indicative Student Workload
Contact Hours
| Full Time | Laboratory
| 30 | Lectures
| 10 |
Directed Study
| | Case Studies
| 20 |
Private Study
| |
| 90 |
Mode of Delivery
This is a laboratory-based course supplemented with lectures and directed self-study exercises.
Assessment Plan
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Learning Outcomes Assessed
| | Component 1 | 1,2
| | Component 2 | 3
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Component 2 is assessed through the submission of a written piece of coursework.
Component 1 is assessed through the submission of written laboratory reports and through the assessment of competence in the laboratory.
Indicative Bibliography
| 1. | FRESHNEY, R.I., 2010. Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications. 6th ed.: Wiley-Blackwell.
| | 2. | DAVIS, J.M., 2002. Basic Cell Culture: A Practical Approach. 2nd ed: Oxford University Press.
| | 3. | REED, R., HOLMES, D., WEYERS, J. AND JONES, A., 2007. Practical Skills in Biomolecular Sciences. 3rd ed.: Pearson Education Ltd.
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